What is the catalog number for MSH6 Antibody?

The catalog number for MSH6 Antibody is orb2642520.

What sizes are available for MSH6 Antibody?

MSH6 Antibody is available in the following size(s): 100 µg.

Is MSH6 Antibody in stock?

MSH6 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

MSH6 Antibody

MSH6 is a component of the post-replicative DNA mismatch repair system (MMR) . Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. [UniProt]

Product Specifications

UniProt

P52701

Reactivity

Human

Immunogen

A portion of amino acids 374-540 from the human protein were used as the immunogen for this MSH6 antibody.

Clonality

Monoclonal

Clone

MTS6-1

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The stated application concentrations are suggested starting points. Titration of the MSH6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.