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Phospho-c-Jun Antibody (pT91/93)

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [RefSeq]

Product Specifications

UniProt

P05412

Reactivity

Human

Immunogen

The amino acids surrounding phosphorylated T91 and T93 were used as the immunogen for the phospho-c-Jun antibody.

Clonality

Monoclonal

Clone

C-J 4C4/1

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Western blot: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the phospho-c-Jun antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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