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Recombinant Ep-CAM Antibody / Rabbit Monoclonal

Ep-CAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Product Specifications

UniProt

P16422

Reactivity

Human

Immunogen

Human protein was used as the immunogen for the recombinant Ep-CAM antibody.

Clonality

Recombinant

Clone

EGP40/1556R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein A affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the recombinant Ep-CAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P

Host or Source

Rabbit

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Rabbit IgG, kappa

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