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CD20 Antibody

CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. It is expressed by pre B-cells and persists during all stages of maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

Product Specifications

UniProt

P11836

Reactivity

Human

Immunogen

Recombinant CD20 protein was used as the immunogen for this CD20 antibody. The epitope is known to be extracellular.

Clonality

Monoclonal

Clone

CDLA20-1

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

FACS: 0.5-1ug/million cells, Immunofluorescence: 0.5-1ug/ml, Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the CD20 antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Tested Applications

FACS, IF, IHC-P

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2a, kappa

Frequently Asked Questions

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