What is the catalog number for EMI-1 Antibody?

The catalog number for EMI-1 Antibody is orb2641717.

What sizes are available for EMI-1 Antibody?

EMI-1 Antibody is available in the following size(s): 7 mL.

Is EMI-1 Antibody in stock?

EMI-1 Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

EMI-1 Antibody

Early Mitotic Inhibitor-1 regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . It is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, EMI-1 is transcriptionally induced by the E2F transcription factor. Overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of EMI-1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.

Product Specifications

UniProt

Q9UKT4

Reactivity

Human

Immunogen

A human partial recombinant N-terminal protein fragment was used as the immunogen for the EMI-1 antibody.

Clonality

Monoclonal

Clone

FBXP5

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the EMI-1 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.