CD45RA Antibody

CD45RA is an isoform of the human leukocyte common antigen (CD45) . Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells.

Product Specifications

UniProt

P08575

Reactivity

Human

Immunogen

Recombinant human protein was used as immunogen for this anti-CD45RA antibody.

Clonality

Monoclonal

Clone

CDLA45RA-1

Conjugation

Unconjugated

Purification

Protein G affinity chromatography

Dilution

Western blot: 1-2ug/ml, Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 ug/ml, Immunohistology (FFPE) : 0.5-1.0ug/ml for 30 min at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Titering of the anti-CD45RA antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.