GITR Antibody (TNFRSF18)
GITR (glucocorticoid-induced TNFR-related gene) is a member of the TNF-receptor superfamily, also known as TNFRSF18. It is expressed at low levels on resting T lymphocytes and at high levels on CD25+ CD4+ Tregs. The expression of GITR on T cells can be upregulated upon activation. Interaction of GITR with its ligand (GITRL) has been demonstrated to augment T cell activation, proliferation, cytokine production as well as MAPKs and NF-kB activation, and abrogate the inhibitory function of CD25+ CD4+ Tregs. In vivo activation of GITR causes development of autoimmune diseases and restores the suppressed immune response.
Product Specifications
UniProt
O35714
Reactivity
Mouse
Immunogen
Mouse CD25+ CD4+ T cells were used as the immunogen for the GITR antibody.
Clonality
Monoclonal
Clone
DTA-1
Conjugation
Unconjugated
Field of Research
Cell Biology
Purification
Protein G affinity chromatography
Dilution
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the GITR antibody should be determined by the researcher.
Tested Applications
FACS, IF
Host or Source
Rat
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rat IgG2b, lambda
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