What is the catalog number for EGFR Antibody / Epidermal Growth Factor Receptor?

The catalog number for EGFR Antibody / Epidermal Growth Factor Receptor is orb2638361.

What sizes are available for EGFR Antibody / Epidermal Growth Factor Receptor?

EGFR Antibody / Epidermal Growth Factor Receptor is available in the following size(s): 100 µg.

Is EGFR Antibody / Epidermal Growth Factor Receptor in stock?

EGFR Antibody / Epidermal Growth Factor Receptor is currently made to order / not held in immediate stock. Contact us for lead time.

EGFR Antibody / Epidermal Growth Factor Receptor

This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Product Specifications

UniProt

P00533

Reactivity

Human

Immunogen

Human EGF Receptor purified from A431 cells was used as the immunogen for the EGFR antibody.

Clonality

Monoclonal

Clone

31G7

Conjugation

Unconjugated

Field of Research

Stem Cell & Developmental Biology; Cell Biology

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 2-4ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the EGFR antibody should be determined by the researcher.1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.