What is the catalog number for AFP Antibody Cocktail?

The catalog number for AFP Antibody Cocktail is orb2638343.

What sizes are available for AFP Antibody Cocktail?

AFP Antibody Cocktail is available in the following size(s): 100 µg.

Is AFP Antibody Cocktail in stock?

AFP Antibody Cocktail is currently made to order / not held in immediate stock. Contact us for lead time.

AFP Antibody Cocktail

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP) . This mAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Product Specifications

UniProt

P02771

Reactivity

Human

Immunogen

Recombinant full-length human Alpha Fetoprotein was used as the immunogen for the AFP antibody cocktail.

Clonality

Monoclonal

Clone

C2 + C3 + MBS-12

Conjugation

Unconjugated

Field of Research

Cancer Biology

Purification

Protein G affinity chromatography

Dilution

Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the AFP antibody cocktail should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.