CD45RB Antibody

CD45RB is one of the four major isoforms of CD45. The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. Clone PD7/26 antibody is specific to CD45RB, and stains most leukemias and lymphomas. This CD45RB antibody is often used in a cocktail with clone 2B11, which will bind to all CD45 isoforms.

Product Specifications

Reactivity

Human

Immunogen

Human peripheral blood lymphocytes maintained in T cell growth factor were used as the immunogen for this CD45RB antibody.

Clonality

Monoclonal

Clone

PD7/26

Conjugation

Unconjugated

Purification

Protein G purified

Dilution

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1ug/ml, Western blot: 1-2ug/ml, Immunohistochemistry (FFPE) : 0.25-0.5ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Variations in protocols, secondaries and substrates may require the CD45RB antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.