What is the catalog number for NCAM Antibody / CD56?

The catalog number for NCAM Antibody / CD56 is orb2637861.

What sizes are available for NCAM Antibody / CD56?

NCAM Antibody / CD56 is available in the following size(s): 100 µg.

Is NCAM Antibody / CD56 in stock?

NCAM Antibody / CD56 is currently made to order / not held in immediate stock. Contact us for lead time.

NCAM Antibody / CD56

This antibody reacts with an extracellular domain (close to transmembrane) of NCAM (Neural cell adhesion molecule), also called CD56. Three isoforms of NCAM / CD56 are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. NCAM antibody recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas) . It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma) . NCAM antibody plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.

Product Specifications

Reactivity

Human, Rat

Immunogen

A membrane preparation of a small cell lung carcinoma was used as the immunogen for this CD56 / NCAM antibody.

Clonality

Monoclonal

Clone

123C3.D5

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Purification

Protein G affinity chromatography

Dilution

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM EDTA Buffer, pH 9, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.