EGFR Antibody / EGF Receptor
EGFR (Epidermal growth factor receptor, HER1, ErbB1) is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain.
Product Specifications
UniProt
P00533
Reactivity
Human
Immunogen
Purified EGF Receptor protein from A431 cells was used as the immunogen for the EGFR antibody.
Clonality
Monoclonal
Clone
225
Conjugation
Unconjugated
Field of Research
Cancer Biology; Cell Biology
Purification
Protein A/G affinity
Dilution
Immunofluorescence: 1-3ug/ml, Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the EGFR antibody should be determined by the researcher.
Tested Applications
ELISA, IF, WB
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG2b, kappa
Available Sizes
Frequently Asked Questions
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