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HLA-ABC Antibody (MHC I)

Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C) . Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.

Product Specifications

UniProt

P30443, P30499, P01889

Reactivity

Human

Immunogen

Human peripheral blood leukocytes were used as the immunogen for the HLA-ABC antibody.

Clonality

Monoclonal

Clone

SPM419

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation; Cell Biology

Purification

Protein A/G affinity

Dilution

Flow cytometry: 1-2ug/million cells, Western blot: 1-2ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the HLA-ABC antibody should be determined by the researcher.

Tested Applications

FACS, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG2a, kappa

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