Myf-4 Antibody / MYOG / Myogenin
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6) . MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif.MyoD family members share about 80% amino acid homology in their bHLH motifs.Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
Product Specifications
UniProt
P15173
Reactivity
Human
Immunogen
Human recombinant protein was used as the immunogen for the Myf-4 antibody.
Clonality
Monoclonal
Clone
PCRP-MYOG-1C5
Conjugation
Unconjugated
Field of Research
Stem Cell & Developmental Biology
Purification
Protein A/G affinity
Dilution
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml, Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the Myf-4 antibody should be determined by the researcher.
Tested Applications
ELISA, FACS, IF, WB
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2b, kappa
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
delta Sarcoglycan (SGCD) (NM_000337) Human Over-expression Lysate
