PARP12 Antibody

Goat polyclonal to PARP12

Product Specifications

Product Name Alternative

Anti PARP12 antibody, anti poly (ADP-ribose) polymerase family, member 12 antibody, anti HGNC:21919 antibody, anti FLJ22693 antibody, anti PARP-12 antibody, anti ZC3H1 antibody, anti ZC3HDC1 antibody, anti zinc finger CCCH type domain containing 1 antibody, anti zinc finger CCCH-type domain containing 1 antibody

Reactivity

Human

Target

PARP12

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

NAHDIKNKSSAP

Field of Research

Signal Transduction

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.03 μg/ml

Molecular Weight

79.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Preliminary experiments gave an approx 110kDa band in Human Liver lysates and in lysates of hepatoblastoma cell line HEPG2 after 0.03 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 79.1kDa according to NP_073587.1. The 110kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Canine

Tested Applications

ELISA, IF

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.