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Smooth muscle alpha-actin Antibody

Goat polyclonal antibody to ACTA2

Product Specifications

Product Name Alternative

Anti-a actin antibody, anti-AAT6 antibody, anti-ACTA_HUMAN antibody, anti-ACTA2 antibody, anti-Actin alpha 2 smooth muscle aorta antibody, anti-Actin aortic smooth muscle antibody, anti-Actin, aortic smooth muscle antibody, anti-ACTSA antibody, anti-ACTVS antibody, anti-Alpha 2 actin antibody, anti-Alpha actin 2 antibody, anti-Alpha cardiac actin antibody, anti-alpha sma antibody, anti-Alpha-actin-2 antibody

Reactivity

Human, Mouse, Rat

Target

Smooth muscle alpha-actin

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EEEDSTALVC

Field of Research

Musculoskeletal & Connective Tissue Research

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:128000, WB: 0.3-1 μg/ml

Molecular Weight

42

References & Citations

Nagy, Bence M. et al. Importance of kynurenine in pulmonary hypertension Am J Physiol Lung Cell Mol Physiol, 313, L741-L751 (2017)

Pubmed id

28705908

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: Successful IHC-P data obtained from a previous batch. An anonymous customer found positive results in IHC-P on Pig heart tissue.WB: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1) . Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: see publication McIntire RH et al., Placenta. 2008 Jul;29 (7) :631-8. Epub 2008 Jun 5. (PMID: 18538388) .

Prediction Reactivity

Canine

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Entrez

59

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