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WHIP/WRNIP1 Antibody

Goat polyclonal antibody to WRNIP1

Product Specifications

Product Name Alternative

Anti WHIP antibody, anti Werner helicase interacting protein antibody, anti FLJ22526 antibody, anti putative helicase RUVBL antibody, anti Werner helicase interacting protein 1 antibody, anti RP11-420G6.2 antibody, anti bA420G6.2 antibody

Reactivity

Human

Target

WHIP / WRNIP1

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

EELRGVDFFKQRRC

Field of Research

Epigenetics & Chromatin

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 4-6μg/ml

Molecular Weight

72.1; 69.5

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 45kDa band in human lung and brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of 72kDa for isoform 1 according to NP_064520.2 and 69.5kDa for isoform 2 according to NP_569079.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

Prediction Reactivity

Bovine, Canine, Mouse, Rat

Tested Applications

ELISA, IHC, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

56897

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