WHIP/WRNIP1 Antibody
Goat polyclonal antibody to WRNIP1
Product Specifications
Product Name Alternative
Anti WHIP antibody, anti Werner helicase interacting protein antibody, anti FLJ22526 antibody, anti putative helicase RUVBL antibody, anti Werner helicase interacting protein 1 antibody, anti RP11-420G6.2 antibody, anti bA420G6.2 antibody
Reactivity
Human
Target
WHIP / WRNIP1
Clonality
Polyclonal
Conjugation
Unconjugated
Sequence
EELRGVDFFKQRRC
Field of Research
Epigenetics & Chromatin
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Dilution
ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 4-6μg/ml
Molecular Weight
72.1; 69.5
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Preliminary experiments gave an approx 45kDa band in human lung and brain lysates after 1 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted sizes of 72kDa for isoform 1 according to NP_064520.2 and 69.5kDa for isoform 2 according to NP_569079.1. The 45kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
Prediction Reactivity
Bovine, Canine, Mouse, Rat
Tested Applications
ELISA, IHC, WB
Preservative
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Entrez
56897
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