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Goat anti-RNF35 / TRIM40 Antibody

Goat polyclonal to RNF35

Product Specifications

Product Name Alternative

Anti TRIM40 antibody, anti tripartite motif-containing 40 antibody, anti RNF35 antibody, anti ring finger RNF35 antibody, anti DAAP-200B17.3 antibody, anti ring finger protein 40 antibody

Reactivity

Human

Target

RNF35 / TRIM40

Clonality

Polyclonal

Conjugation

Unconjugated

Sequence

GVSELLLQPPQKL

Field of Research

Cell Biology

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Dilution

ELISA: 1:32000, WB: 0.5-2 μg/ml

Molecular Weight

26.1

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx. 45kDa band observed in Human Brain lysates at 0.5-1ug of primary, this band was successfully blocked by incubation with the immunising peptide. Please note that the band we observe is different from the predicted size of approx. 26kDa according to NP_619645. Currently we cannot find an explanation for this in the literature. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any splice variants/modified forms been reported

Tested Applications

ELISA, WB

Preservative

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Entrez

135644

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