Anti-6 His Antibody
Rabbit Polyclonal Antibody specific to 6 His
Product Specifications
CAS Number
9007-83-4
Product Name Alternative
Anti-6 His Rabbit Polyclonal Antibody
Host
Rabbit
Reactivity
6 His
Immunogen
6-His (HHHHHH) conjugated to KLH.
Target
6 His
Clonality
Polyclonal
Isotype
Whole IgG
Type
Antibody
Applications
WB, IP, ICC, ELISA
Field of Research
Epitope Tags
Purification Method
Purified by peptide immuno-affinity chromatography
Concentration
1.0 mg/mL
Dilution
Dilute in PBS or medium that is identical to that used in the assay system.
Format
Purified
Form
Liquid
Buffer
Phosphate Buffered Saline
Additionnal Information
Immunoblotting: use at a dilution of 1:1,000-1:20,000.
<br><br>ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500.
<br><br>Immunocytochemistry: use at a dilution of 1:100-1:400.
<br><br>Immunoprecipitation: use at 1-4ug/mg lysate
<br><br>These are recommended dilutions.
<br><br>Endusers should determine optimal dilutions for their applications.
Storage Conditions
This antibody is stable for at least one (1) year at 2-8°C.
Specificity
Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Formulation
Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide
Buffer pH
pH 7.2
Target Background
A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
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