Anti-6 His Antibody

Rabbit Polyclonal Antibody specific to 6 His

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-6 His Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

6 His

Immunogen

6-His (HHHHHH) conjugated to KLH.

Target

6 His

Clonality

Polyclonal

Isotype

Whole IgG

Type

Antibody

Applications

WB, IP, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000-1:20,000. ELISA: for detection, use at a dilution of 1:1,000-1:30,000; for coating, use at a dilution of 1:100- 1:500. Immunocytochemistry: use at a dilution of 1:100-1:400. Immunoprecipitation: use at 1-4ug/mg lysate These are recommended dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.

Formulation

Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide

Buffer pH

pH 7.2

Target Background

A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.