3X FLAG peptide
3X FLAG peptides are FLAG-tagged peptides containing three repeats of the Asp-Tyr-Lys-Xaa-Xaa-Asp motif. 3X FLAG peptide can be used for protein separation and purification, and competitive elution with target proteins.
Product Specifications
CAS Number
402750-12-3
UNSPSC
12352209
Target
Biochemical Assay Reagents
Type
Peptides
Related Pathways
Others
Field of Research
Others
Assay Protocol
https://www.medchemexpress.com/3X_FLAG_peptide.html
Purity
99.97
Solubility
DMSO : 10 mg/mL (ultrasonic) |H2O : 33.33 mg/mL (ultrasonic; adjust pH to 2 with 1 M HCL)
Smiles
OC(C=C1)=CC=C1C[C@H](NC([C@H](CC(O)=O)NC([C@H]([C@@H](C)CC)NC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CC(O)=O)NC([C@H](CCCCN)NC([C@@H](NC([C@H](CC(O)=O)NC(CNC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CC(O)=O)NC([C@H](CCCCN)NC([C@@H](NC([C@H](CC(O)=O)NC([C@@H](N)CCSC)=O)=O)CC2=CC=C(C=C2)O)=O)=O)=O)CC3=CN=CN3)=O)=O)=O)=O)CC4=CC=C(C=C4)O)=O)=O)=O)CC5=CN=CN5)=O)=O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O
Molecular Formula
C120H169N31O49S
Molecular Weight
2861.87
References & Citations
[1]Zilio N, et al. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017 Mar 1;2017 (3) :pdb.prot091611.|[2]Gao XK, et al. Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discov. 2022 Jun 28;8 (1) :60. |[3]Cong M, et al. MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination. Nat Cancer. 2020 Feb;1 (2) :222-234.
Shipping Conditions
Blue Ice
Storage Conditions
-80°C, 2 years; -20°C, 1 year (Powder, sealed storage, away from moisture)
Scientific Category
Peptides
Clinical Information
No Development Reported
Citation 01
BioRxiv. 2025 April 28.|bioRxiv. 2025 March 25.|bioRxiv. 2025 Sep 7.|Cell Discov. 2022 Jun 28;8 (1) :60.|Cell Discov. 2023 Aug 1;9 (1) :83.|Cell Res. 2025 Jun;35 (6) :437-452.|Complutense University of Madrid. 2025.|Int J Biol Macromol. 2025 Jan 7:139619.|J Biol Chem. 2024 Jul 14:107575.|J Integr Plant Biol. 2022 Sep;64 (9) :1789-1802.|J Mol Biol. 2024 Jun 20:168672.|Nat Commun. 2025 Sep 2;16 (1) :8182.|Nature Cancer. 222-234 (2020) .|Res Sq. 2025 Aug 31.|Technical University of Darmstadt. 2025 Oct 8.|bioRxiv. 2023 Nov 4.|Cell Death Dis. 2025 Aug 7;16 (1) :595.|Int J Mol Sci. 2025 Mar 29;26 (7) :3159.|J Adv Res. 2025 Aug:74:225-236.|Nat Microbiol. 2021 May;6 (5) :658-671.
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