3X FLAG peptide

3X FLAG peptides are FLAG-tagged peptides containing three repeats of the Asp-Tyr-Lys-Xaa-Xaa-Asp motif. 3X FLAG peptide can be used for protein separation and purification, and competitive elution with target proteins.

Product Specifications

CAS Number

[402750-12-3]

UNSPSC

12352209

Target

Biochemical Assay Reagents

Type

Peptides

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/3X_FLAG_peptide.html

Purity

99.97

Solubility

DMSO : 10 mg/mL (ultrasonic) |H2O : 33.33 mg/mL (ultrasonic; adjust pH to 2 with 1 M HCL)

Smiles

OC(C=C1)=CC=C1C[C@H](NC([C@H](CC(O)=O)NC([C@H]([C@@H](C)CC)NC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CC(O)=O)NC([C@H](CCCCN)NC([C@@H](NC([C@H](CC(O)=O)NC(CNC([C@H](CC(O)=O)NC([C@@H](NC([C@H](CC(O)=O)NC([C@H](CCCCN)NC([C@@H](NC([C@H](CC(O)=O)NC([C@@H](N)CCSC)=O)=O)CC2=CC=C(C=C2)O)=O)=O)=O)CC3=CN=CN3)=O)=O)=O)=O)CC4=CC=C(C=C4)O)=O)=O)=O)CC5=CN=CN5)=O)=O)=O)=O)C(N[C@@H](CCCCN)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@@H](CC(O)=O)C(N[C@H](C(O)=O)CCCCN)=O)=O)=O)=O)=O)=O

Molecular Formula

C120H169N31O49S

Molecular Weight

2861.87

References & Citations

[1]Zilio N, et al. Improved Tandem Affinity Purification Tag and Methods for Isolation of Proteins and Protein Complexes from Schizosaccharomyces pombe. Cold Spring Harb Protoc. 2017 Mar 1;2017 (3) :pdb.prot091611.|[2]Gao XK, et al. Phase separation of insulin receptor substrate 1 drives the formation of insulin/IGF-1 signalosomes. Cell Discov. 2022 Jun 28;8 (1) :60. |[3]Cong M, et al. MTSS1 suppresses mammary tumor-initiating cells by enhancing RBCK1-mediated p65 ubiquitination. Nat Cancer. 2020 Feb;1 (2) :222-234.