Anti-c-myc Antibody

Rabbit Polyclonal Antibody specific to c-myc

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-c-myc Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

C-Myc

Immunogen

Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.

Target

c-myc

Clonality

Polyclonal

Type

Antibody

Applications

WB, IP, ICC, ELISA

Field of Research

Epitope Tags

Purification Method

Purified by peptide affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at a dilution of 1:1,000- 1:25,000 Immunocytochemistry: use at a dilution of 1:100-1:400. Immunofluorescence: use at a dilution of 1:100-1:400 ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100-1:500. These are recommended dilutions. End users should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at 2-8°C.

Specificity

c-myc

Formulation

PBS, pH 7.2, 0.09% sodium azide.

Buffer pH

pH 7.2

Target Background

c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.