Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)

Product Specifications

Product Name Alternative

EF-Tu

Abbreviation

Recombinant Xanthomonas oryzae pv. oryzae tuf protein

Gene Name

Tuf

UniProt

A0A0J9WWH0

Expression Region

1-384aa

Organism

Xanthomonas oryzae pv. oryzae (strain PXO99A)

Target Sequence

MNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALDGDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIKVGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVKMTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK

Tag

N-terminal 10xHis-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Epigenetics and Nuclear Signaling

Relevance

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

47.7 kDa

References & Citations

"Genome sequence and rapid evolution of the rice pathogen Xanthomonas oryzae pv. oryzae PXO99A." Salzberg S.L., Sommer D.D., Schatz M.C., Phillippy A.M., Rabinowicz P.D., Tsuge S., Furutani A., Ochiai H., Delcher A.L., Kelley D., Madupu R., Puiu D., Radune D., Shumway M., Trapnell C., Aparna G., Jha G., Pandey A. Bogdanove A.J. BMC Genomics 9:204-204 (2008)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.