Recombinant Xanthomonas oryzae pv. oryzae Elongation factor Tu (tuf)
Product Specifications
Product Name Alternative
EF-Tu
Abbreviation
Recombinant Xanthomonas oryzae pv. oryzae tuf protein
Gene Name
Tuf
UniProt
A0A0J9WWH0
Expression Region
1-384aa
Organism
Xanthomonas oryzae pv. oryzae (strain PXO99A)
Target Sequence
MNVGTIGHVDHGKTTLTAALTKIGAERFGGEFKAYDAIDAAPEEKARGITISTAHVEYESPSRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPHIVVFLNKADMVDDAELLELVEMEVRELLSKYDFPGDDTPIIHGSARLALDGDQSEIGVPAILKLVDALDTFIPEPTRDVDRPFLMPVEDVFSISGRGTVVTGRIERGIIKVGDEIEIVGIRATQKTTVTGVEMFRKLLDQGQAGDNAGLLLRGTKRDDVERGQVLCKPGSIKPHTEFEAEVYVLSKDEGGRHTPFFKGYRPQLYFRTTDITGAIDLPEGVEMVMPGDNVKMTVTLINPVAMDEGLRFAIREGGRTVGAGVVSKIIK
Tag
N-terminal 10xHis-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Epigenetics and Nuclear Signaling
Relevance
This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
47.7 kDa
References & Citations
"Genome sequence and rapid evolution of the rice pathogen Xanthomonas oryzae pv. oryzae PXO99A." Salzberg S.L., Sommer D.D., Schatz M.C., Phillippy A.M., Rabinowicz P.D., Tsuge S., Furutani A., Ochiai H., Delcher A.L., Kelley D., Madupu R., Puiu D., Radune D., Shumway M., Trapnell C., Aparna G., Jha G., Pandey A. Bogdanove A.J. BMC Genomics 9:204-204 (2008)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Curated Selection
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