Recombinant Shigella dysenteriae serotype 1 Shiga toxin subunit B (stxB)
Product Specifications
Product Name Alternative
StxB; SDY_1390; Shiga toxin subunit B
Abbreviation
Recombinant Shigella dysenteriae serotype 1 stxB protein
Gene Name
StxB
UniProt
Q32GM0
Expression Region
21-89aa
Organism
Shigella dysenteriae serotype 1 (strain Sd197)
Target Sequence
TPDCVTGKVEYTKYNDDDTFTVKVGDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Signal Transduction
Relevance
The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide in human intestinal microvilli.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
The B subunit is responsible for the binding of the holotoxin to specific receptors on the target cell surface, such as globotriaosylceramide (Gb3) in human intestinal microvilli.
Molecular Weight
15.1 kDa
References & Citations
"Genome dynamics and diversity of Shigella species, the etiologic agents of bacillary dysentery." Yang F., Yang J., Zhang X., Chen L., Jiang Y., Yan Y., Tang X., Wang J., Xiong Z., Dong J., Xue Y., Zhu Y., Xu X., Sun L., Chen S., Nie H., Peng J., Xu J. Jin Q. Nucleic Acids Res. 33:6445-6458 (2005)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS1426381-01 0.02 mg (E-Coli)
Recombinant Yersinia pseudotuberculosis Imidazole glycerol phosphate synthase subunit HisH (hisH)
Ask
View Details 
MBS1426381-02 0.1 mg (E-Coli)
Recombinant Yersinia pseudotuberculosis Imidazole glycerol phosphate synthase subunit HisH (hisH)
Ask
View Details MBS1426381-03 0.02 mg (Yeast)
Recombinant Yersinia pseudotuberculosis Imidazole glycerol phosphate synthase subunit HisH (hisH)
Ask
View Details MBS1426381-04 0.1 mg (Yeast)
Recombinant Yersinia pseudotuberculosis Imidazole glycerol phosphate synthase subunit HisH (hisH)
Ask
View Details 
MBS1426381-05 0.02 mg (Baculovirus)
Recombinant Yersinia pseudotuberculosis Imidazole glycerol phosphate synthase subunit HisH (hisH)
Ask
View Details 
MBS1426381-06 0.02 mg (Mammalian-Cell)
Recombinant Yersinia pseudotuberculosis Imidazole glycerol phosphate synthase subunit HisH (hisH)
Ask
View Details