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Recombinant Aspergillus flavus Exochitosanase, partial

Product Specifications

Product Name Alternative

Exo-beta-D-glucosaminidase

Abbreviation

Recombinant Aspergillus flavus Exochitosanase protein, partial

UniProt

P83488

Expression Region

1-17aa

Organism

Aspergillus flavus

Target Sequence

LPTGPNNPTTLDNSSII

Tag

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Exohydrolysis of beta-1,4-linkages between N-acetyl-D-glucosamine and D-glucosamine residues, and D-glucosamine and D-glucosamine residues in chitosan.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Exohydrolysis of beta-1,4-linkages between N-acetyl-D-glucosamine and D-glucosamine residues, and D-glucosamine and D-glucosamine residues in chitosan.

Molecular Weight

17.8 kDa

References & Citations

"Purification and characterization of the exo-beta-D-glucosaminidase from the fungus, A. flavus IAM2044." Ji J.-H., Hur J.-W. J. Microbiol. Biotechnol. 13:269-275 (2003)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

Available Sizes

Frequently Asked Questions

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