Recombinant Treponema pallidum 34 kDa membrane antigen (tpd)

Product Specifications

Product Name Alternative

Pathogen-specific membrane antigen

Abbreviation

Recombinant Treponema pallidum tpd protein

Gene Name

Tpd

UniProt

P19478

Expression Region

20-204aa

Organism

Treponema pallidum (strain Nichols)

Target Sequence

CGGGGEHQHGEEMMAAVPAPDAEGAAGFDEFPIGEDRDVGPLHVGGVYFQPVEMHPAPGAQPSKEEADCHIEADIHANEAGKDLGYGVGDFVPYLRVVAFLQKHGSEKVQKVMFAPMNAGDGPHYGANVKFEEGLGTYKVRFEIAAPSHDEYSLHIDEQTGVSGRFWSEPLVAEWDDFEWKGPQW

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Cancer

Relevance

This antigen is a pathogen-specific membrane immunogen.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

This antigen is a pathogen-specific membrane immunogen.

Molecular Weight

27.1 kDa

References & Citations

"The binary protein interactome of Treponema pallidum--the syphilis spirochete." Titz B., Rajagopala S.V., Goll J., Hauser R., McKevitt M.T., Palzkill T., Uetz P. PLoS ONE 3:e2292-e2292 (2008)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.