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Recombinant Bacillus cereus UV DNA damage endonuclease (uvsE)

Product Specifications

Product Name Alternative

UvsE; BCG9842_B5482; UV DNA damage endonuclease; UV-endonuclease; UVED; EC 3.-.-.-

Abbreviation

Recombinant Bacillus cereus uvsE protein

Gene Name

UvsE

UniProt

B7IR00

Expression Region

1-317aa

Organism

Bacillus cereus (strain G9842)

Target Sequence

MIMRFGYVSHAMALWDCSPAKTMTFTSFKKLSKQEREDKLYHVIRQNLEHTIRILHYNIAHEIPLYRLSSSIVPLATHPEVEFDYIGVFTPLWRKIGALIKEHNLRISFHPNQFTLFTSDKPHITTNAITDMTYHYKILDAIGIADSSYINIHVGGAYGNKEKAIERFHENIKKLPAHIKKQMTLENDDKTYTTSETLSICQKENIPFVFDYHHHMANLCEQPLEELLPAIFETWSHTNISPKVHISSPRSEKEFRAHAEYIDLEFIKPFLHVAKKNNHNFDIMIESKQKDLALFRLIDELSAIRGIKRISGAMLQW

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Component in a DNA repair pathway. Removal of UV LIGHT damaged nucleotides. Recognizes pyrimidine dimers and cleave a phosphodiester bond immediately 5' to the lesion (By similarity) .

Molecular Weight

44.0 kDa

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length

Available Sizes

Curated Selection

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