Recombinant Treponema phagedenis Treponemal membrane protein B (tmpB)
Product Specifications
Product Name Alternative
Antigen TmpB
Abbreviation
Recombinant Treponema phagedenis tmpB protein
Gene Name
TmpB
UniProt
P29720
Expression Region
22-384aa
Organism
Treponema phagedenis
Target Sequence
ISYDDNEYSRKSRAYTQLAEKAYDAGEYDTAIEYSQLAENFAQESAEYIKRMMARNEAEDAMNKARTRYAWAKQQKADKNYPTEYLIAGEAIKAGGIAFDNKNYDVAVTCAEKALESLKTVEPEDKVIAKAAADKAAAEKAAKEKAAREKSAKDKAAKEKAAKEKAAKDKAAKEKAAKEKAAKDKAAKEKAAKEKAAREMAAKEKAAKDKAAKEEAARKAAEEAAARKAAEEAAARKAAEEEAARIAAEEEAARKAAEEEAARKAAEEALYNEKGEKVLPSEYKVLTWKLDRECFWNIAKNPAVYNDPFMWRKLYEANKDKIPESNNPDWVEAETILVIPSIRGERREGLYDPDVKYQALPKR
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Tmp may serve as a porin or transport protein for large molecules.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Tmp may serve as a porin or transport protein for large molecules.
Molecular Weight
47.4 kDa
References & Citations
"Treponema phagedenis encodes and expresses homologs of the Treponema pallidum TmpA and TmpB proteins." Yelton D.B., Limberger R.J., Curci K., Malinosky-Rummell F., Sliviensky L., Schouls L.M., van Embden J.D.A., Charon N.W. Infect. Immun. 59:3685-3693 (1991)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Frequently Asked Questions
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