Recombinant Mouse Ig kappa chain V-V region K2
Product Specifications
Product Name Alternative
Ig kappa chain V-V region K2; Fragment
Abbreviation
Recombinant Mouse Ig kappa chain V-V region K2 protein
UniProt
P01635
Expression Region
21-115aa
Organism
Mus musculus (Mouse)
Target Sequence
DIQMTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYNAKTLADGVPSRFSGSGSGTQYSLKINSLQPEDFGSYYCQHFWSTP
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
The gene was isolated and sequenced separately from two different sources, embryos and cultured plasmacytoma cells that secrete the similar kappa chain MOPC 149.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
17.4 kDa
References & Citations
"Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis." Seidman J.G., Leder A., Edgell M.H., Polsky F., Tilghman S.M., Tiemeier D.C., Leder P. Proc. Natl. Acad. Sci. U.S.A. 75:3881-3885 (1978)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Frequently Asked Questions
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