Recombinant Pig Saposin-B-Val (PSAP)

Product Specifications

Product Name Alternative

Cerebroside sulfate activator (CS-ACT) (Non-specific activator) (Sphingolipid activator protein 1) (SAP-1)

Abbreviation

Recombinant Pig PSAP protein

Gene Name

PSAP

UniProt

P81405

Expression Region

1-80aa

Organism

Sus scrofa (Pig)

Target Sequence

GDVCQDCIQMVTDLQNAVRTNSTFVEALVNHAKEECDRLGPGMADMCKNYISQYSEIAIQMMMHMQPKDICGLVGFCEEV

Tag

N-terminal 6xHis-Flag-tagged

Type

In Stock Protein

Source

Yeast

Field of Research

Metabolism

Relevance

Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22) . Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Molecular Weight

11.9 kDa

References & Citations

"Porcine cerebroside sulfate activator: further structural characterization and disulfide identification." Stevens R.L., Faull K.F., Conklin K.A., Green B.N., Fluharty A.L. Biochemistry 32:4051-4059 (1993)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.