Recombinant Streptomyces globisporus Lysozyme M1 (acm)
Product Specifications
Product Name Alternative
1,4-beta-N-acetylmuramidase M1
Abbreviation
Recombinant Streptomyces globisporus acm protein
Gene Name
Acm
UniProt
P25310
Expression Region
78-294aa
Organism
Streptomyces globisporus
Target Sequence
DTSGVQGIDVSHWQGSINWSSVKSAGMSFAYIKATEGTNYKDDRFSANYTNAYNAGIIRGAYHFARPNASSGTAQADYFASNGGGWSRDNRTLPGVLDIEHNPSGAMCYGLSTTQMRTWINDFHARYKARTTRDVVIYTTASWWNTCTGSWNGMAAKSPFWVAHWGVSAPTVPSGFPTWTFWQYSATGRVGGVSGDVDRNKFNGSAARLLALANNTA
Tag
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Others
Relevance
This enzyme has both lysozyme (acetylmuramidase) and diacetylmuramidase activities.
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
This enzyme has both lysozyme (acetylmuramidase) and diacetylmuramidase activities.
Molecular Weight
30.6 kDa
References & Citations
"A new lysozyme fold. Crystal structure of the muramidase from Streptomyces coelicolor at 1.65 A resolution." Rau A., Hogg T., Marquardt R., Hilgenfeld R. J. Biol. Chem. 276:31994-31999 (2001)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
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