Recombinant Danio rerio Hydroxylysine kinase (hykk)

Product Specifications

Product Name Alternative

Agphd1

Abbreviation

Recombinant Zebrafish hykk protein

Gene Name

Hykk

UniProt

A8WFT6

Expression Region

1-355aa

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Target Sequence

MSTKESKPNLSHSQVTDVVKRLYGLTASVVRPLPSYDDQNFYVAPSEGGEFVLKVMNSADSENVAVIELQTQSMNFLHQRGLPAQTALPTLTGQLMSLEEFDCGFGTQIYLVRLLTYLPGTTIAKITCSPQILYDVGKMAATLDTVLLQMEHPNTRVLQRERFIWKLTSIPLLNQYVHVMDGDPVQKIVKGVIEKYQVQVMPKLPLFRECINHGDFNDHNLLVKPDGPSKYKISGILDFADMSCGYFIFELAITIMYMMIESPTPLDVGGPVVAGWESVFPLNEAERDSLYWLVMCRFCQSLVLARYTVIQQPENEEYLMITSRKGLRHLSRLWELGKDEVERRWFQSAQQFRQI

Tag

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Cell Biology

Relevance

Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Catalyzes the GTP-dependent phosphorylation of 5-hydroxy-L-lysine.

Molecular Weight

60.5 kDa

References & Citations

NIH - Zebrafish Gene Collection (ZGC) project Submitted (NOV-2007)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.