Recombinant Pseudomonas putida Arylalkyl acylamidase

Product Specifications

Product Name Alternative

Arylalkyl acylamidase; EC 3.5.1.76; Fragment

Abbreviation

Recombinant Pseudomonas putida Arylalkyl acylamidase protein

UniProt

P35902

Expression Region

1-28aa

Organism

Pseudomonas putida (Arthrobacter siderocapsulatus)

Target Sequence

TRRTAFLFDELSLWHSASQYALILPVGG

Tag

Tag-Free

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Shows a strict specificity for N-acetyl arylalkylamines but not acetanilide derivatives.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Shows a strict specificity for N-acetyl arylalkylamines but not acetanilide derivatives.

Molecular Weight

3.1 kDa

References & Citations

"Purification and characterization of a novel enzyme, arylalkyl acylamidase, from Pseudomonas putida Sc2." Shimizu S., Ogawa J., Chung M.C.-M., Yamada H. Eur. J. Biochem. 209:375-382 (1992)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.