Recombinant Plasmodium falciparum Erythrocyte-binding antigen 175, partial

Product Specifications

Product Name Alternative

Erythrocyte-binding antigen 175; EBA-175

Abbreviation

Recombinant Plasmodium falciparum Erythrocyte-binding antigen 175 protein, partial

UniProt

P19214

Expression Region

634-833aa

Organism

Plasmodium falciparum (isolate Camp / Malaysia)

Target Sequence

GDDYCQDKTKMIETLKVECKEKPCEDDNCKSKCNSYKEWISKKKEEYNKQAKQYQEYQKGNNYKMYSEFKSIKPEVYLKKYSEKCSNLNFEDEFKEELHSDYKNKCTMCPEVKDVPISIIRNNEQTSQEAVPEENTEIAHRTETPSISEGPKGNEQKERDDDSLSKISVSPENSRPETDAKDTSNLLKLKGDVDISMPKA

Tag

N-terminal 10xHis-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Binds to host erythrocytes; may act as bridge between erythrocytes and merozoites.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Binds to host erythrocytes; may act as bridge between erythrocytes and merozoites.

Molecular Weight

28.7 kDa

References & Citations

"Primary structure of the 175K Plasmodium falciparum erythrocyte binding antigen and identification of a peptide which elicits antibodies that inhibit malaria merozoite invasion." Sim B.K., Orlandi P.A., Haynes J.D., Klotz F.W., Carter J.M., Camus D., Zegans M.E., Chulay J.D. J. Cell Biol. 111:1877-1884 (1990)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.