Recombinant Acinetobacter calcoaceticus Catechol 1,2-dioxygenase

Product Specifications

Product Name Alternative

Catechol 1,2-dioxygenase; EC 1.13.11.1; Fragment

Abbreviation

Recombinant Acinetobacter calcoaceticus Catechol 1,2-dioxygenase protein

UniProt

P83715

Expression Region

1-14aa

Organism

Acinetobacter calcoaceticus

Target Sequence

MNSQQIDMLVKQLN

Tag

N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Others

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Molecular Weight

21.7 kDa

References & Citations

"Growth on phenol at chemostress levels amplifies the expression of the phenol degradation pathway in Acinetobacter calcoaceticus." Benndorf D., Loffhagen N., Hartig C., Babel W. Eng. Life Sci. 4:38-42 (2004)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.