Recombinant Rat Thyrotropin subunit beta (Tshb)

Product Specifications

Product Name Alternative

Thyroid-stimulating hormone subunit beta

Abbreviation

Recombinant Rat Tshb protein

Gene Name

Tshb

UniProt

P04652

Expression Region

21-132aa

Organism

Rattus norvegicus (Rat)

Target Sequence

FCIPTEYMMYVDRRECAYCLTINTTICAGYCMTRDINGKLFLPKYALSQDVCTYRDFTYRTVEIPGCPHHVAPYFSYPVALSCKCGKCNTDYSDCTHEAVKTNYCTKPQTFY

Tag

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Signal Transduction

Relevance

Indispensable for the control of thyroid structure and metabolism.

Endotoxin

Not test

Purity

Greater than 85% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Indispensable for the control of thyroid structure and metabolism.

Molecular Weight

19.9 kDa

References & Citations

"Thyroid hormone decreases thyrotropin subunit mRNA levels in rat anterior pituitary." Croyle M.L., Maurer R.A. DNA 3:231-236 (1984)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.