Recombinant Human SH3 domain-containing YSC84-like protein 1 (SH3YL1)
Product Specifications
Product Name Alternative
DKFZP586F1318; RAY; SH3 domain containing; Ysc84-like 1 (S. cerevisiae) ; SH3 domain-containing YSC84-like protein 1; SH3Y1_HUMAN; Sh3yl1
Abbreviation
Recombinant Human SH3YL1 protein
Gene Name
SH3YL1
UniProt
Q96HL8
Expression Region
1-342aa
Organism
Homo sapiens (Human)
Target Sequence
MNNPIPSNLKSEAKKAAKILREFTEITSRNGPDKIIPAHVIAKAKGLAILSVIKAGFLVTARGGSGIVVARLPDGKWSAPSAIGIAGLGGGFEIGIEVSDLVIILNYDRAVEAFAKGGNLTLGGNLTVAVGPLGRNLEGNVALRSSAAVFTYCKSRGLFAGVSLEGSCLIERKETNRKFYCQDIRAYDILFGDTPRPAQAEDLYEILDSFTEKYENEGQRINARKAAREQRKSSAKELPPKPLSRPQQSSAPVQLNSGSQSNRNEYKLYPGLSSYHERVGNLNQPIEVTALYSFEGQQPGDLNFQAGDRITVISKTDSHFDWWEGKLRGQTGIFPANYVTMN
Tag
N-terminal 6xHis-tagged and C-terminal Myc-tagged
Type
Developed Protein
Source
Yeast
Field of Research
Cell Biology
Endotoxin
Not test
Purity
Greater than 85% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Molecular Weight
40.6 kDa
References & Citations
"SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain." Hasegawa J., Tokuda E., Tenno T., Tsujita K., Sawai H., Hiroaki H., Takenawa T., Itoh T. J. Cell Biol. 193:901-916 (2011)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
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