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Recombinant Taraxacum officinale 2S albumin, partial

Product Specifications

Product Name Alternative

To-A1

Abbreviation

Recombinant Taraxacum officinale 2S albumin protein, partial

UniProt

P86783

Expression Region

1-19aa

Organism

Taraxacum officinale (Common dandelion) (Leontodon taraxacum)

Target Sequence

PVSRQQCSQRIQGERFNQC

Tag

Tag-Free

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

This is a 2S seed storage protein. Has antifungal activity. Inhibits spore germination in H.sativum (IC50=62.5 µg/ml) and P.betae (IC50=62.5 µg/ml) . Inhibits growth of H.sativum, V.albo-atrum and P.infestans.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

This is a 2S seed storage protein. Has antifungal activity. Inhibits spore germination in H.sativum (IC (50) =62.5 ug/ml) and P.betae (IC (50) =62.5 ug/ml) . Inhibits growth of H.sativum, V.albo-atrum and P.infestans.

Molecular Weight

2.3 kDa

References & Citations

"Antifungal activity of storage 2S albumins from seeds of the invasive weed dandelion Taraxacum officinale Wigg." Odintsova T.I., Rogozhin E.A., Sklyar I.V., Musolyamov A.K., Kudryavtsev A.M., Pukhalsky V.A., Smirnov A.N., Grishin E.V., Egorov T.A. Protein Pept. Lett. 17:522-529 (2010)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Partial

Available Sizes

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