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Recombinant Mouse Alpha-defensin 1 (Defa1)

Product Specifications

Product Name Alternative

Cryptdin-1 Defensin-related cryptdin peptide

Abbreviation

Recombinant Mouse Defa1 protein

Gene Name

Defa1

UniProt

P11477

Expression Region

59-93aa

Organism

Mus musculus (Mouse)

Target Sequence

LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR

Tag

N-terminal GST-tagged

Type

Developed Protein

Source

Yeast

Field of Research

Immunology

Relevance

Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.

Molecular Weight

31.1 kDa

References & Citations

"Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium." Ouellette A.J., Greco R.M., James M., Frederick D., Naftilan J., Fallon J.T. J. Cell Biol. 108:1687-1695 (1989)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Protein Length

Full Length of Mature Protein

Available Sizes

Frequently Asked Questions

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