Recombinant Mouse Alpha-defensin 1 (Defa1)
Product Specifications
Product Name Alternative
Cryptdin-1 Defensin-related cryptdin peptide
Abbreviation
Recombinant Mouse Defa1 protein
Gene Name
Defa1
UniProt
P11477
Expression Region
59-93aa
Organism
Mus musculus (Mouse)
Target Sequence
LRDLVCYCRSRGCKGRERMNGTCRKGHLLYTLCCR
Tag
N-terminal GST-tagged
Type
Developed Protein
Source
Yeast
Field of Research
Immunology
Relevance
Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Probably contributes to the antimicrobial barrier function of the small bowel mucosa. Has antibacterial activity against attenuated mutants of S.typhimurium.
Molecular Weight
31.1 kDa
References & Citations
"Developmental regulation of cryptdin, a corticostatin/defensin precursor mRNA in mouse small intestinal crypt epithelium." Ouellette A.J., Greco R.M., James M., Frederick D., Naftilan J., Fallon J.T. J. Cell Biol. 108:1687-1695 (1989)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length of Mature Protein
Available Sizes
Curated Selection
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