Recombinant Plasmodium falciparum Apical membrane antigen 1 (AMA-1), partial
Product Specifications
Product Name Alternative
Merozoite surface antigen
Abbreviation
Recombinant Plasmodium falciparum AMA-1 protein, partial
Gene Name
AMA-1
UniProt
P22621
Expression Region
42-73aa&227-271aa&346-407aa&452-488aa&525-546aa
Organism
Plasmodium falciparum (isolate FC27 / Papua New Guinea)
Target Sequence
NEHREHPKEYQYPLHQEHTYQQEDSGEDENTLDNDKNSNYKYPAVYDDKDKKCHILYIAAQENNGPRYCNKDESKRNASDQPKQYEQHLTDYEKIKEGFKNKNASMIKSAFLPTGAFKADRYKSHGKGYNWGNYNTETQKEIERESKRIKLNDNDDEGNKKIIAPRIFISDDKDSLKEEYKDEYADIPEHKPTYDKMK
Tag
N-terminal 6xHis-tagged
Type
In Stock Protein
Source
E.coli
Field of Research
Others
Relevance
Involved in parasite invasion of erythrocytes.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Involved in parasite invasion of erythrocytes.
Molecular Weight
27.5 kDa
References & Citations
"Integral membrane protein located in the apical complex of Plasmodium falciparum." Peterson M.G., Marshall V.M., Smythe J.A., Crewther P.E., Lew A., Silva A., Anders R.F., Kemp D.J. Mol. Cell. Biol. 9:3151-3154 (1989)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Partial
Available Sizes
Curated Selection
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