Recombinant Bacillus subtilis Stage V sporulation protein M (spoVM)

Product Specifications

Product Name Alternative

SpoVM; BSU15810; Stage V sporulation protein M

Abbreviation

Recombinant Bacillus subtilis spoVM protein

Gene Name

SpoVM

UniProt

P37817

Expression Region

1-26aa

Organism

Bacillus subtilis (strain 168)

Target Sequence

MKFYTIKLPKFLGGIVRAMLGSFRKD

Tag

Tag-Free

Type

Developed Protein

Source

E.coli

Field of Research

Others

Relevance

Required for normal formation of the cortex and coat during sporulation in bacillus subtilis where it plays a morphogenetic rather than a regulatory role.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Required for normal formation of the cortex and coat during sporulation in bacillus subtilis where it plays a morphogenetic rather than a regulatory role.

Molecular Weight

3 kDa

References & Citations

"A 28 kbp segment from the spoVM region of the Bacillus subtilis 168 genome." Foulger D., Errington J. Microbiology 144:801-805 (1998)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.