AMPPD

AMPPD (Lumi-Phos Plus; Lumigen PPD) is a chemiluminescent substrate for alkaline phosphatase (APase) . AMPPD is hydrolyzed by APase to generate an unstable dioxetane intermediate, and the intermediate releases a chemiluminescent signal when it decomposes. The luminescent signal of AMPPD can be detected by highly sensitive equipment, thereby achieving quantitative analysis of the target molecule. AMPPD can be used in ultrasensitive enzyme-linked immunosorbent assays (such as quantitative detection of human tissue kininogen), chemiluminescent detection of proteins and nucleic acids, and other fields[1][2].

Product Specifications

CAS Number

[122341-56-4]

Product Name Alternative

Lumi-Phos Plus; Lumigen PPD; PPD

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Phosphatase

Type

Biochemical Assay Reagents

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/AMPPD.html

Concentration

10mM

Purity

99.93

Solubility

H2O : 175 mg/mL (ultrasonic)

Smiles

COC1(C2=CC(OP(O)(O)=O)=CC=C2)C3(C(C4)CC5CC4CC3C5)OO1

Molecular Formula

C18H23O7P

Molecular Weight

382.34

Precautions

H302, H315, H319, H335

References & Citations

[1]Legris F, et al. An ultrasensitive chemiluminoenzyme immunoassay for the quantification of human tissue kininogens: application to synovial membrane and cartilage. J Immunol Methods. 1994 Jan 12;168 (1) :111-21.|[2]Green MR, et al. Preparation of Labeled DNA, RNA, and Oligonucleotide Probes. Cold Spring Harb Protoc. 2022 Jan 4;2022 (1) .