Recombinant Human Kinetochore-associated protein DSN1 homolog (DSN1)
Product Specifications
Product Name Alternative
C20orf172; Chromosome 20 open reading frame 172; dJ469A13.2; DSN1; DSN1; MIND kinetochore complex component; homolog; DSN1; MIND kinetochore complex component; homolog (S. cerevisiae) ; DSN1; MIS12 kinetochore complex component; DSN1_HUMAN; FLJ13346; hKNL-3; Kinetochore associated protein DSN1; Kinetochore associated protein DSN1 homolog; Kinetochore null 3 homolog; Kinetochore-associated protein DSN1 homolog; KNL3; MGC32987; MIS13
Abbreviation
Recombinant Human DSN1 protein
Gene Name
DSN1
UniProt
Q9H410
Expression Region
1-356aa
Organism
Homo sapiens (Human)
Target Sequence
MTSVTRSEIIDEKGPVMSKTHDHQLESSLSPVEVFAKTSASLEMNQGVSEERIHLGSSPKKGGNCDLSHQERLQSKSLHLSPQEQSASYQDRRQSWRRASMKETNRRKSLHPIHQGITELSRSISVDLAESKRLGCLLLSSFQFSIQKLEPFLRDTKGFSLESFRAKASSLSEELKHFADGLETDGTLQKCFEDSNGKASDFSLEASVAEMKEYITKFSLERQTWDQLLLHYQQEAKEILSRGSTEAKITEVKVEPMTYLGSSQNEVLNTKPDYQKILQNQSKVFDCMELVMDELQGSVKQLQAFMDESTQCFQKVSVQLGKRSMQQLDPSPARKLLKLQLQNPPAIHGSGSGSCQ
Tag
N-terminal GST-tagged
Type
Developed Protein
Source
E.coli
Field of Research
Cell Biology
Relevance
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
Endotoxin
Not test
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Form
Liquid or Lyophilized powder
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Function
Part of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
Molecular Weight
67.1 kDa
References & Citations
"A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1." Obuse C., Iwasaki O., Kiyomitsu T., Goshima G., Toyoda Y., Yanagida M. Nat. Cell Biol. 6:1135-1141 (2004)
Storage Conditions
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Protein Length
Full Length
Available Sizes
Curated Selection
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