Recombinant Human BH3-like motif-containing cell death inducer (BLID)

Product Specifications

Product Name Alternative

Breast cancer cell protein 2

Abbreviation

Recombinant Human BLID protein

Gene Name

BLID

UniProt

Q8IZY5

Expression Region

1-108aa

Organism

Homo sapiens (Human)

Target Sequence

MVTLLPIEGQEIHFFEILESECVLYTGWIERASGSSIYPEAKARLPLEALLGSNKEPMLPKETVLSLKRYNLGSSAMKRNVPGHVLQRPSYLTRIQVTLLCNSSAEAL

Tag

N-terminal GST-tagged

Type

Developed Protein

Source

E.coli

Field of Research

Cell Biology

Relevance

Functions as a proapoptotic molecule through the caspase-dependent mitochondrial pathway of cell death.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Functions as a proapoptotic molecule through the caspase-dependent mitochondrial pathway of cell death.

Molecular Weight

39 kDa

References & Citations

"BRCC2, a novel BH3-like domain-containing protein, induces apoptosis in a caspase-dependent manner." Broustas C.G., Gokhale P.C., Rahman A., Dritschilo A., Ahmad I., Kasid U. J. Biol. Chem. 279:26780-26788 (2004)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.