Recombinant Chicken anemia virus Apoptin (VP3)

Product Specifications

Product Name Alternative

VP3; Apoptin

Abbreviation

Recombinant Chicken anemia virus VP3 protein

Gene Name

VP3

UniProt

P54095

Expression Region

1-120aa

Organism

Chicken anemia virus (isolate USA 26p4) (CAV)

Target Sequence

MNALQEDTPPGPSTVFRPPTSSRPLETPHCEIRIGIAGITITLSLCGCANARAPTLRSATADSSESTGFKNAPDLRTDQPKPPSKKRSCDPSEYRVSELKENLITTTPSRPRTARRCIRL

Tag

N-terminal 6xHis-tagged

Type

In Stock Protein

Source

E.coli

Field of Research

Microbiology

Relevance

May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle.

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

May act as transcriptional regulator. Induces apoptosis in infected cells. Element of infectious replication cycle.

Molecular Weight

17.1 kDa

References & Citations

"Molecular cloning and sequence analysis of the genome of chicken anaemia agent." Claessens J.A.J., Schrier C.C., Mockett A.P.A., Jagt E.H.J.M., Sondermeijer P.J.A. J. Gen. Virol. 72:2003-2006 (1991)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.