Recombinant Hepatitis E virus genotype 3 Pro-secreted protein ORF2 (ORF2), partial

Product Specifications

Product Name Alternative

Protein ORF2

Abbreviation

Recombinant Hepatitis E virus genotype 3 ORF2 protein, partial

Gene Name

ORF2

UniProt

Q9YLQ9

Expression Region

411-660aa

Organism

Hepatitis E virus genotype 3 (isolate Human/United States/US2) (HEV-3)

Target Sequence

KLYTSVENAQQDKGITIPHDIDLGDSRVVIQDYDNQHEQDRPTPSPAPSRPFSVLRANDVLWLSLTAAEYDQTTYGSSTNPMYVSDTVTLVNVATGAQAVARSLDWSKVTLDGRPLTTIQQYSKTFYVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQILIENAAGHRVAISTYTTSLGAGPTSISAVGVLAPHSALAVLEDTIDYPARAHTFDDFCPECRTLGLQGCAFQSTIAELQRLKMKVGKTRES

Tag

N-terminal 6xHis-tagged

Type

In Stock Protein

Source

Yeast

Field of Research

Microbiology

Relevance

Major viral capsid protein that encapsidates the viral genome. Binds to the 5' end of the genomic RNA (By similarity) .

Endotoxin

Not test

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Form

Liquid or Lyophilized powder

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Function

Major viral capsid protein that encapsidates the viral genome. Binds to the 5' end of the genomic RNA (By similarity) .

Molecular Weight

29.3 kDa

References & Citations

"A hepatitis E virus variant from the United States: molecular characterization and transmission in cynomolgus macaques."Erker J.C., Desai S.M., Schlauder G.G., Dawson G.J., Mushahwar I.K.J. Gen. Virol. 80:681-690 (1999)

Storage Conditions

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.